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^CgFDevelopment of a multiplex polymerase chain reaction assay for detection and
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M^CgFiɂ<I>Staphylococcus aureus</I>̌oƋʂ̂߂̑dPCR@̊J
ҁFTamarapu S, Drake M, Mckillip J L
sNAŁFJournal of  Food Protection@i2001j, <B>64</B>, (5), 664]668 
ێF1.<I>Staphylococcus aureus</I>
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i <I>S.aureus</I>DNA̗LȒo@yѓőfY <I>S.aureus</I>̌oƋʂs߂̑dPCR@J
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ΏێB1)lדIɉEу`F_[`[Y@2)gpۊ@<I>S.aureus</I> LT1ALT2AATCC12600(ent C)A3862(ent C)A13565(ent A)A13566(ent A)A14458(ent B)721̋ہB
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<ITEM5>
 
1)DNAoB܂̓`[Yt10ml100%G^m[2mlAZ_AjE2mlAΖG[e2ml悭A12000xgA10.SsB̒a300l10mMgX[_ɏՉtpH7.8ɌB10llysostaphini5mg/mljA37A5|{B50l10%SDSA5ԃ{CBʂ̃NzA17000xgA10SBtŵA2.5M|_AtipH5.4j0.1̐ρA100G^m[2.5̐ρAOR[Qi10mg/mlj5lADNA𒾓aADNASi20000xgA30jADNAگĂ30l10mMTris-EDTAɏՉtpH7.2ōpB
2)PCRvC}[BőfY<I>S.aureus</I>oɔM萫kNA[[`q(nuc)IԁBnucentCiHegLVC`qj̃vC}[V[PXWilson炩瓾BvC}[͊OB
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4)dPCR@entC nuc̗`qWIɂAdPCR{BoDNA@2.5lAW`XzILTCh1lA25mM}OlVE2lAie`qɑ΂jtH[hvC}[уo[XvC}[e20pmol,PCRX[p[~bNX44lAAj[Ox56ȊO͏L@ƓB
5)yfBPCRYmFRFLP@\zB3̐yf@AluTARsaTAHsp92UBPCRY5lA12.3lŋېA10~yfɏՉt2.3lAyf0.5lA372ԕێB𕨂1%VQŕLG`WEŐFUVŉB
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Journal of Food Protection, 64, 664
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<PUBMED>

http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?form=4&db=m&Term=Journal of Food Protection, 64, 664

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