ԍFs-58
^CgFMultiplex polymerase chain reaction assay for simultaneous detection of
@@@@@<I>Staphylococcus aureus</I> and Streptococcal causes of bovine mastitis
M^CgFEV[̌<I>Staphylococcus aureus</I>Streptococcus𓯎o邽߂
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ҁFPHUEKTES P, MANSELL P D, BROWNING G F
sNAŁFJournal of Dairy Science@(2001), <B>84</B>, (5), 1140]1148
ێF1.<I>Staphylococcus aureus</I>
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<ITEM1> \n̖ړI
 
4̎ȓ[ہi<I>S.aureus</I>,<I>S.agalactiae</I>.S.dysgalactiae,<I>S.uberis</I>j̓o@ƂāA16S23S rRNAXy[T[̈̑ɊÂdPCRJB
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I[XgASouth Gippsland̐݊Q̎悵117̃~N
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<ITEM3>
 
̎Oɓ70%G^m[ZЖȕzłʂBn߂͎̓̂āA5ml̓ŋۃvX`bNeɍ̂B͍̎36Ԉȓ -20œ͎{܂ŕۑB
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<ITEM4> \n̐
 
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<ITEM5>
 
1)ۊBRg[<I>S.aureus</I>,<I>S.agalactiae</I>.S.dysgalactiaeA<I>S.uberis</I>͓[늳番B
2)ISkNI`hvC}[BSẴISkNI`hvC}[͌\ꂽV[PXɂBJvC}[͕\1ɎB
3)PCRƑdPCRِ̓BPCRvC}[ِ̓́AɕʂɌێi<I>S.epidermidis</I>A<I>S.intermedius</I>AEnterococcus@faecalisA<I>E.coli</I>AS.bovisAS.zooepidemicusA<I>M.bovis</I>jŒׂB
4)PCRƑdPCR̊xB<I>S.aureus</I>A<I>S.agalactiae</I>AS.dysgalactiaeA<I>S.uberis</I>̐DNA10{ߗpĊePCR@̊x肵B
5)PCRx̌B<I>S.aureus</I>A<I>S.agalactiae</I>AS.dysgalactiaeA<I>S.uberis</I>pāAPCR̊xƊeo@̊x𒲂ׂB
6)DNÃtFm[-NzoB300l̓300l0.5%SDS100l/ml̃veCi[[K܂NTEot@[i0.1MNacl,20mMgX-_[pH7.4],1mMEDTA[pH7.4]jƍA374ԃCLx[gAʂ̃tFm[ENzEC\A~AR[i25:24:1j3ɁA10,000xg 3ԉSwW߂B̑2JԂBwW60l3M|_Na(pH4.8)1.2ml̗100%G^m[A-2030ێDNA𒾓bB410,000xg15SDNAA70%G^m[Ő􂢁A10,000xg5ԎŉSBDNA͕50lTEobt@[ŗnB
7)DNÃXsJoBe100l1%2-JvgG^m[܂350lRLTobt@[ƌ300l100%@@G^m[15lQiaexUmatrixBXsJɃAvCA9,000xgA30bSB600lRLTobt@[ŃJ􂢁A500lRPEobt@[2􂤁i9,000xg30bSjB16,000xgA1.5SAߏ̃G^m[B30lRNaset[}gbNXɒڂ̂A9,000xgA1SDNAnoB
8)PCRÝ̕@@@Y12l0.1g/mlLG`WE܂2%VQœdCji0.5xTPEobt@[6V/cm@45jBPCRY͎OŉAY7l20%PAGÉi6V/cm2ԓdCjFjAqʃ}[J[ɂ̓vX~hpU18HaeVGhkNA[[𕨂50bpXebv_[pB
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<ITEM6>
 
dPCR̊x͏]@̔|{@茟oxႩADNAȏOɔZk邱Ƃɂ芴xǂꂽBjQ߂ꂽƂыېƂɂB̌ʁA<I>S.aureus</I>,<I>S.uberis</I>̌o͏]@xȂA<I>S.agalactiae</I>,S.dysgalactiaěox͏]@ƓłBdPCR@́A`4ۂ̓ov,xɂłA[`̐ff@ɗpłƎvꂽ
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<ITEM7>
 
Journal of Dairy Science, 84,1140
@
<PUBMED>

http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?form=4&db=m&Term=Journal of Dairy Science, 84,1140

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s-58-1.gif
