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^CgFComparison of different approaches to quantify <I>Staphylococcus aureus</I> cells by real]time
@@@@@ quantitative PCR and application of this technique for examination of cheese
M^CgFA^CPCRɂ<I>Staphylococcus aureus</I>ۑ̂ʂۂ̈قȂ@̔r
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ҁFHEIN I, LEHNER A, RIECK P, BRANDL E, WAGNER M, KLEIN K
sNAŁFApplied and environmental microbiology@i2001j, <B>67</B>, (7),  3122]3126 
ێF1.<I>Staphylococcus aureus</I>
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<ITEM1> \n̖ړI
 
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<ITEM4> \n̐
 
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<ITEM5>
 
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3)TaqManv[ugpLightCyclerVXeyABI PRISM 7700@SDSVXeBTaqManv[ugpRTQ-PCŔALightCycler-DNA Master Hybridization v[uLbgA邢LightCycler@-FastStart DNA Maste Hybridization v[uLbgpB
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5)lדI`[YDNAPB2g̃`[YA12jbg/ml̃vi[[܂1:10ߏɏՉtɉXg}bJ[1ώA40@3ԕێA45,700xg15SsybgTEɏՉt3APCRɏՉt1򂷂Bybg25li`Wbg`[Yj邢250liJ}x[`[YjPCRɏՉtɗnA143jbg/ml̃]X^tBB15ێ8jbg/ml̃veCi[[KA601ԕێǍ9515ێ13,000xg5SsȀ㐴5lRTQ-PCRɋB
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nuc`q269bpfЂARTQ-PCRɂ<I>S.aureus</I>זĚoyђʗp̕WIƂđI񂾁BSYBR@GreenIgpLightCyclerVXeɂRTQ]PCRsƂC̊xTaqManv[ugpꍇɔׂĒႢƂ킩BTaqManv[ugpALightCyclerVXeɂ銴xČ́AABIPrism7700@SDSVXepꍇƂقړłB TaqManv[u]LightCyclervgRlHI<I>S.aureus</I>`[Y̌ɓKpB̌ʁA1.5xE+2`6.4xE+2Rs[/2g̊xnuc`qoł邱ƁACFȖΐlnucRs[̑֌W0D979`0D998ł邱Ƃ킩B ̌ʂARTQ]PCR`[Yɂ<I>S.aureus</I>̒ʂɗpł邱ƂB
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<ITEM7>
 
Applied and Environmental Microbiology, 67, 3122
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<PUBMED>

http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?form=4&db=m&Term=Applied and Environmental Microbiology, 67, 3122

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